| Autor: |
Seman, Gabriel |
| Zdroj: |
Journal of Tissue Culture Methods; 1980, Vol. 6 Issue 3/4, p141-143, 3p |
| Abstrakt: |
A method for propagating cells of established human breast cancer lines in floating aggregates is described. The cells are grown in stationary cultures using conventional T25 and T75 plastic flasks inlaid with a base of solid agar. The cells aggregate by mutual recognition alone. This culture method has the advantages of high cell yields and of easy handling for subculture. No scraping or trypsinization is required. Above all the method has the merit of revealing, by light and by electron microscopy, the degree of differentiation and other characteristics of the cells not seen in monolayers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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