| Autor: |
Yamanaka, Sumie, Hashimoto, Masanori, Tobe, Masuo, Kobayashi, Kazuo, Sekizawa, Jun, Nishimura, Masao |
| Zdroj: |
Archives of Toxicology; Jun1990, Vol. 64 Issue 4, p262-268, 7p |
| Abstrakt: |
We proposed a simple method for screening assessment of acute oral and dermal toxicity using only three rats and mice of each sex at each dose level. Animals were first treated with chemicals at a dose of 2000 mg/kg and were carefully observed for compound-related morbidity and mortality. If none of the animals died, the following toxicity tests were suspended. If some of the animals died, toxicity tests at doses of 200 and 20 mg/kg were performed. The approximate LD values calculated by this method showed little difference between two separate laboratories and were in good agreement with LD values reported in the literature. Our toxicological data also showed that LD values were about 2-2.5 times the MNLD (maximum non lethal dose) in acute oral and dermal toxicity. This meant that a chemical could be regarded as having an LD of about 4000 mg/kg or higher when there was no mortality at the dose of 2000 mg/kg. A chemical with such low toxicity would not require further testing for lethal effects. Therefore, this simple method combining the fixed-dose procedure with the limit test is suitable for determination of approximate LD values of chemicals and for screening for necessity for classical full LD test using many animals. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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