Cloning and sequencing of a ligninase gene from a lignindegrading basidiomycete, Phanerochaete chrysosporium.

Autor: Asada, Yasuhiko, Kimura, Yoshio, Kuwahara, Masaaki, Tsukamoto, Akira, Koide, Kazuo, Oka, Atsuhiro, Takanami, Mitsuru
Zdroj: Applied Microbiology & Biotechnology; 1988, Vol. 29 Issue 5, p469-473, 5p
Abstrakt: A ligninase gene has been cloned from a Phanerochaete chrysosporium genomic DNA library. Nucleotide sequencing of the gene has revealed that the ligninase structural gene contains 1116 bp of the protein-encoding sequence, of which 84 bp encode the signal peptide. The protein-encoding sequence is interrupted by eight introns which conform to the universal G-T/A-G splicing rule observed for the 3′ and 5′ intron boundaries. The putative eukaryotic regulatory sequences, i.e. 'CAAT' and 'TATA' box-like sequences, are present in the 5′ flanking region. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index