| Autor: |
Old, Jennifer, Schweers, Brett A., Boonlayangoor, Pravat W., Fischer, Brian, Miller, Kevin W. P., Reich, Karl |
| Předmět: |
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| Zdroj: |
Journal of Forensic Sciences; Mar2012, Vol. 57 Issue 2, p489-499, 11p |
| Abstrakt: |
Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate-specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID™-Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID™-Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross-react with other human or nonhuman tissues tested. RSID™-Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA-based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID™-Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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