Mapping of Marek's disease virus genome: Identification of junction sequences between unique and inverted repeat regions.

Autor: Makimura, Koichi, Peng, Fang-Yu, Tsuji, Miho, Hasegawa, Saori, Kawai, Yuri, Nonoyama, Meihan, Tanaka, Akiko
Zdroj: Virus Genes; Jan1994, Vol. 8 Issue 1, p15-24, 10p
Abstrakt: Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TR/U, U/IR, IR/U, and U/TR. The TR/U junction is located 192 bp downstream of the last EcoRI site in the TR region, while the U/IR junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IR region. The IR/U junction is located 950 bp downstream of the second EcoRI site in the IR region, while the U/TR junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TR region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U and -P, upstream of the U/TR junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TR/U junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the U/IR region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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