Cancer patients' lymphocytes contain CD3CD4cells that proliferate in response to autologous tumor cells in the presence of exogenous low-dose interleukin-2 and autologous accessory cells.

Autor: Radrizzani, Marina, Quaia, Michele, Benedetti, Barbara, Andreola, Salvatore, Vaglini, Maurizio, Galligioni, Enzo, Fossati, Giuseppe, Parmiani, Giorgio
Zdroj: Cancer Immunology, Immunotherapy; Jul1989, Vol. 30 Issue 4, p233-238, 6p
Abstrakt: To see whether cancer patients possess CD3 CD4 lymphocytes able to proliferate in response to autologous tumor cells (Auto-Tu), this lymphocyte subset was isolated either by positive or negative selection, both methods resulting in highly enriched CD4 populations. Unseparated and isolated CD3 CD4 lymphocytes were then assayed for proliferating activity in the presence or absence of various amounts of Auto-Tu, with or without recombinant interleukin-2 (IL-2) (1.5-15 U/ml) and DR adherent cells or E lymphocytes as autologous accessory cells (Auto-AC). Isolated CD3 CD4 lymphocytes were stimulated by Auto-Tu alone in only 1 out of 12 cases. CD3 CD4 cells failed to proliferate significantly in response to low doses of IL-2 alone but the addition of Auto-Tu caused stimulation in 8 out of 12 cases (67%). The further addition of Auto-AC to Auto-Tu + IL-2 resulted in enhanced response of isolated CD3 CD4 lymphocytes in 6 out of 8 cases tested. When reactivities to Auto-Tu in the presence of IL-2 and IL-2 + Auto-AC were considered together, positive responses of CD3 CD4 lymphocytes were seen in 11 out of 12 cases (92%). On the other hand, unseparated lymphocytes were stimulated by Auto-Tu alone in none out of 12 cases. Unseparated lymphocytes, however, responded to IL-2 in 11 out of 12 cases; such a response was increased by the addition of Auto-Tu in only 2 cases. Moreover, the IL-2 proliferation of unseparated lymphocytes was suppressed in 4 and in 3 out of 12 cases tested when Auto-Tu or Auto-Tu + Auto-AC were added respectively. These data indicate that lymphocytes of cancer patients contain CD3 CD4 cells that are usually unable to proliferate in response to Auto-Tu only. This proliferation, however, occurs when low doses of exogenous IL-2 are present and can be further amplified by the addition of Auto-AC. No response of CD4 cells is observed in the presence of DR Auto-AC + IL-2 except in 2 out of 7 cases tested (28%), suggesting an Auto-Tu-restricted reactivity of CD3 CD4 lymphocytes in the majority of cases. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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