T cell cytotoxicity of autologous and allogeneic lymphocytes in a patient with Crohn's disease.

Autor: Okazaki, Kazuichi, Yokoyama, Yuichi, Yamamoto, Yasutake, Kobayashi, Michiya, Araki, Keijiro, Ogata, Takuro, Okazaki, K, Yokoyama, Y, Yamamoto, Y, Kobayashi, M, Araki, K, Ogata, T
Zdroj: Journal of Gastroenterology; Aug1994, Vol. 29 Issue 4, p415-422, 8p
Abstrakt: We report a 27-year-old male with Crohn's disease (CD) of the small and large intestine, whose peripheral blood lymphocytes (PBL) showed increased cell-mediated cytotoxicity (CTL). Autologous and allogeneic effector cells from PBL and intestinal lymph nodes (LN) were isolated on a Ficoll-Hypaque gradient. Colonic cells were prepared as the target and were incubated for 6h with effector cells, after being labeled with Na(2)51CrO4. The CTL activity [effector/target (E/T) ratio, 100:1] of PBL for autologous targets was increased by 38% compared with that in normal subjects (< 10%), while that shown by LN was not increased (14%). The CTL activity of allogeneic PBL prepared from three of four other CD patients was also increased. Anti-major histocompatibility (MHC) class I and II and CD4 and CD8 monoclonal antibodies (50 micrograms/ml) significantly inhibited CTL activity. Complement-mediated depletion of CD2+ cells significantly reduced CTL activity. These results suggest that MHC-restricted CTL may play a role in mucosal damage in some patients with Crohn's disease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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