| Autor: |
Fujie, Hitomi, Villena, Julio, Tohno, Masanori, Morie, Kyoko, Shimazu, Tomoyuki, Aso, Hisashi, Suda, Yoshihito, Shimosato, Takeshi, Iwabuchi, Noriyuki, Xiao, Jin-Zhong, Yaeshima, Tomoko, Iwatsuki, Keiji, Saito, Tadao, Numasaki, Muneo, Kitazawa, Haruki |
| Předmět: |
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| Zdroj: |
FEMS Immunology & Medical Microbiology; Oct2011, Vol. 63 Issue 1, p129-139, 11p, 6 Graphs |
| Abstrakt: |
A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEKpTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEKpTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEKpTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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