| Autor: |
Bastonero, Sonia, Gargouri, Myriem, Ortiou, Sandrine, Guéant, Jean-Louis, Merten, Marc D. |
| Zdroj: |
Journal of Gene Medicine; Nov2005, Vol. 7 Issue 11, p1439-1449, 11p |
| Abstrakt: |
Background In vivo, tracheal gland serous cells highly express the cystic fibrosis transmembrane conductance regulator ( cftr) gene. This gene is mutated in the lethal monogenic disease cystic fibrosis (CF). Clinical trials in which the human CFTR cDNA was delivered to the respiratory epithelia of CF patients have resulted in weak and transient gene expression. Methods and results As CF is characterized by mucus inspissation, airway infection, and severe inflammation, we tested the hypothesis that inflammation and especially two cytokines involved in the Th1/Th2 inflammatory response, interleukin 4 (IL-4) and TNFα, could inhibit gene transfer efficiency using a model of human CF tracheal gland cells (CF-KM4) and Lipofectamine reagent as a transfection reagent. The specific secretory defects of CF-KM4 cells were corrected by Lipofectamine-mediated human CFTR gene transfer. However, this was altered when cells were pre-treated with IL-4 and TNFα. Inhibition of luciferase reporter gene expression by IL-4 and TNFα pre-treated CF-KM4 cells was measured by activity and real-time RT-PCR. Both cytokines induced similar and synergistic inhibition of transgene expression and activity. This cytokine-mediated inhibition could be prevented by anti-inflammatory agents such as glucocorticoids but not by non-steroidal (NSAI) agents. Conclusions This data suggests that an inflammatory context generated by IL-4 and TNFα can inhibit human CFTR gene transfer in CF tracheal gland cells and that glucocorticoids may have a protecting action. Copyright © 2005 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text