| Autor: |
Chung, Lip Yong, Lo, Mee Wah, Mustafa, Muhammad Rais, Goh, Swee Hock, Imiyabir, Zamrie |
| Zdroj: |
Phytotherapy Research; Mar2009, Vol. 23 Issue 3, p330-334, 5p |
| Abstrakt: |
A 96-well microplate filtration based 5-HT2A receptor-radioligand binding assay was optimized and adopted to carry out a bioassay-guided fractionation of the methanol extract of the leaves of Litsea sessilis. This purification led to the isolation of two compounds identified as (+)-boldine (1) and (+)-dehydrovomifoliol (2). (+)-Boldine binds to 5-HT2A receptors at high concentrations with a Ki value of 2.16 µ m. However, (+)-dehydrovomifoliol showed minimal competitive inhibition on the binding of [3H]ketanserin to the same receptor with a Ki value of 2.06 m m. These results suggest that (+)-boldine influences the activity of 5-HT2A receptors through competitive binding as an agonist or antagonist. Copyright © 2008 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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