The C-terminal cysteine-rich region dictates specific catalytic properties in chimeras of the ectonucleotidases NTPDase1 and NTPDase2.

Autor: Heine, Petra, Braun, Norbert, Sévigny, Jean, Robson, Simon C., Servos, Jörg, Zimmermann, Herbert
Předmět:
Zdroj: European Journal of Biochemistry; Jan2001, Vol. 268 Issue 2, p364, 10p, 6 Black and White Photographs, 2 Diagrams, 2 Charts, 12 Graphs
Abstrakt: Examines the use of C-terminal cysteine-rich region to dictate specific catalytic properties in chimeras of the ectonucleotidases NTPDase1 and NTPDase 2. Expression of cDNAs in CHO cells; Determination of ATP and ADP hydrolysis rates and ADP and AMP formation; Verification of surface expression by immunocytochemistry.
Databáze: Complementary Index