| Autor: |
Yamada, C, Feitosa, WB, Simões, R, Nicacio, AC, Mendes, CM, Assumpção, MEOA, Visintin, JA |
| Předmět: |
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| Zdroj: |
Reproduction in Domestic Animals; Feb2011, Vol. 46 Issue 1, p173-176, 4p |
| Abstrakt: |
The current study examined the protective effects of -glutamine and cytochalasin B during vitrification of immature bovine oocytes. Oocyte vitrification solution (PBS supplemented with 10% FCS, 25% EG, 25% DMSO and 0.5 trehalose) was the vitrification control. Treatments were the addition of 7 μg/ml cytochalasin B, 80 m glutamine or both cytochalasin and glutaminine for 30 s. After warming, oocytes were matured in vitro for 24 h, fixed and stained with Hoechst (33342) for nuclear maturation evaluation. -glutamine improved the vitrified/warmed immature bovine oocytes viability (32.8%), increasing the nuclear maturation rates compared to other treatments and the no treatment vitrified control (17.4%). There was, however, no effect of cytochalasin B on in vitro maturation (14.4%). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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