| Autor: |
Jones, Janet, Patel, Bhartiben N., Skidmore, Christopher J. |
| Zdroj: |
Carcinogenesis; 1988, Vol. 9 Issue 11, p2023-2026, 4p |
| Abstrakt: |
Benzamides are potent inhibitors of nuclear ADP-ribosyl-transferase and have been extensively used to demonstrate the involvement of ADP-ribosylation in cellular function. When permeabilized L1210 cells are treated with 50 μM 3-acetyl-amidobenzamide (3-aab) the enzyme is inhibited. However, when 50 nM 3-aab is used a two-fold stimulation of enzyme activity is produced. This anomalous stimulation is obtained with benzamides and nicotinamides and is correlated with their activity as inhibitors. Strikingly the steady-state level of poly(ADP-ribose) in intact cells is increased by these low levels of inhibitors. The mechanisms of this effect and its consequences for the experimental use of benzamides are discussed. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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