| Autor: |
Tokunaga, Y, Tokunaga, Yukihiko, Shirahase, Hiroyuki, Yamamoto, Etsuko, Inao, Ryou, Hamaguchi, Sugihiro, Kanaji, Kenji, Kitaoka, Akihiro, Yagi, Toshikazu, Tokuka, Atsuo, Ohsumi, Kiyoshi |
| Předmět: |
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| Zdroj: |
Journal of Gastroenterology & Hepatology; Jun2000, Vol. 15 Issue 6, p617-621, 5p, 2 Charts |
| Abstrakt: |
Abstract Background: The rapid urease test and touch cytology have been used for the rapid detection of Helicobacter pylori infection. Recently, a modified rapid urease (MRU) test, which provides results in 20 min has been available on a commercial basis. To date, few reports have evaluated the accuracy of this test. This study evaluated the sensitivity, specificity, and accuracy of the MRU test and touch cytology to detect H. pylori in relation to the density of H. pylori infection determined semi-quantitatively by using immunohistochemical stains. Methods: Biopsy specimens obtained from a total of 60 patients who underwent endoscopy for evaluation of gastroduodenal diseases were studied by using the MRU test, Giemsa stain for touch smear tissue and histological methods. An immunohistochemical stain was used as a standard, and the density of H. pylori infection was graded according to the number of individual bacteria seen as follows: grade 0 = 0; grade 1+ = 1–9; grade 2+ = 10–29; grade 3+ = 30–99; grade 4+ ≥ 100. The severity of gastritis was evaluated histologically in each specimen and compared with the density of H. pylori infection. Results: The MRU test had an overall sensitivity of 73%, specificity of 100% and accuracy of 85%. The Giemsa stain had a sensitivity of 91%, specificity of 100% and accuracy of 95%. The sensitivities of the MRU test and Giemsa stain decreased in mild H. pylori infection. In the MRU test, the sensitivity was 47% when the density of H. pylori infection was 1+, while 80–100% sensitivities were obtained when the densities of infection were ≥ 2+. With the Giemsa stain, the sensitivity was 80% when the density was 1+, while the sensitivity increased to 100% when the densities were ≥ 2+. The severity of gastritis determined by the Rauws scores showed a positive correlation with the density of H. pylori infection as evaluated by immunohistochemical staining. Conclusions: The MRU test had high sensitivity and specificity for moderate to severe H. pylori infection, but it may result in false-negative results in tests for mild infection. As the MRU test has the advantages of shorter incubation times and low cost, a combination of the MRU test and the Giemsa stain for touch cytology may be the most time- and cost-efficient tests in a clinical setting for the diagnosis of H. pylori infection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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