Analog of mRNA, pUUUGUU derivative with an arylazide group at guanosine residue, crosslinks with nucleotides A1823 and A1824 of 18S rRNA in human 80S ribosomes.

Autor: Demeshkina, N., Repkova, M., Ven’yaminova, A., Graifer, D., Karpova, G.
Zdroj: Molecular Biology; Sep2000, Vol. 34 Issue 5, p759-763, 5p
Abstrakt: Affinity modification of human placental 80S ribosomes was performed using an mRNA analog, pUUUGUU derivative containing a 3′-O-methyl group and photoactivated p-azidotetrafluorobenzoyl group at the N7 atom of guanine, in complexes with one or two codon-anticodon interactions formed with participation of Phe-tRNAPhe or Phe-tRNAPhe and Val-tRNAVal (in both cases, the modified codon GUU was at the A site). It was established by the reverse transcription technique that the mRNA analog crosslinks with nucleotides A1823 and A1824 of 18S rRNA in the complexes of both types. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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