Sudan-β-d-glucuronides and their use for the histochemical localization of β-glucuronidase activity in transgenic plants.

Autor: Van der Eycken, E., Terryn, N., Goeman, J. L., Carlens, G., Nerinckx, W., Claeyssens, M., Van der Eycken, J., Van Montagu, M., Brito-Arias, M., Engler, G.
Zdroj: Plant Cell Reports; Oct2000, Vol. 19 Issue 10, p966-970, 5p
Abstrakt: Synthesis of five different Sudan-β- d-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri- O-acetyl-1-trichloroacetimidoyl-α- d-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used for histochemical localization of β-glucuronidase (GUS) activity in transgenic plants ( Petunia hybrida, Arabidopsis thaliana, and Nicotiana tabacum) that contained the GUS reporter system. Because the cleavage of the β-glucuronide results in the liberation of an insoluble Sudan dye, Sudan substrates gave no diffusion artifacts as described for the commonly used 5-bromo-4-chloro-3-indolyl-β- d-glucuronide (X-gluc). A comparison of assays with different Sudan glucuronides and X-gluc demonstrated that the SudanIV variant is a valuable glucuronide substrate for the precise histochemical localization of GUS activity in transgenic plants. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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