| Autor: |
Miley, Helen E., Holden, Darren, Grint, Richard, Best, Leonard, Brown, P. D. |
| Zdroj: |
Pflügers Archiv: European Journal of Physiology; Dec1997, Vol. 435 Issue 2, p227-230, 4p |
| Abstrakt: |
This study investigated regulatory volume increase (RVI) in rat pancreatic β-cells. Volume changes in isolated β-cells were measured by a video-imaging method. Cell shrinkage was induced by exposure to solutions made hypertonic by the addition of 100 mM mannitol. In HEPES-buffered solutions, β-cells exhibited an RVI which was almost completely abolished by 10 μM bumetanide. These data indicate that Na+-2Cl–-K+ cotransporters make a major contribution to RVI in β-cells. In HCO3 –-buffered solutions, however, an RVI was observed in the presence of 10 μM bumetanide. This bumetanide-insensitive component of RVI was inhibited by 100 μM amiloride or 100 μM 4,4’-diisothiocyanatostilbene-2,2’-disulphonic acid (DIDS). These data suggest that, in addition to the Na+-2Cl–-K+ cotransporter, functionally coupled Na+-H+ exchangers and Cl–-HCO3 – exchangers may also contribute to RVI in pancreatic β-cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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