| Autor: |
Rumijowska-Galewicz, A., Ziółkowski, A., Korycka-Machała, M., Sedlaczek, L. |
| Zdroj: |
World Journal of Microbiology & Biotechnology; Apr2000, Vol. 16 Issue 3, p237-244, 8p |
| Abstrakt: |
The rate of transformation of β-sitosterol to 4-androsten-3,17-dione (AD) by Mycobacterium vaccae increased considerably in the presence of D,L-norleucine and m-fluorophenylalanine. These compounds inhibit the biosynthesis of the complex lipids in the cell wall outermost layer. Non-covalently linked (free) lipids were extracted from control and inhibitor-treated cells, and the fatty acid patterns were determined in the neutral lipid, glycolipid and phospholipid fractions. Profound differences were revealed in both the fatty acid composition and the quantities of the individual components in each analysed fraction, derived from the cells exposed to the action of the inhibitors. Partial disorganization of the outermost layer of M. vaccae cell is assumed to alter the cell wall permeability to β-sitosterol and to enhance its biotransformation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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