The expression of vasa gene during zebrafish ( Danio rerio) oogenesis.

Autor: Fang, Xiang, Yan, Zhen, Wen-xuan, Zheng, Feng-jiao, Deng, Xiao-kai, Wang, Xi-yuan, Zhang
Zdroj: Wuhan University Journal of Natural Sciences; Nov2004, Vol. 9 Issue 6, p979-982, 4p
Abstrakt: vasa gene expression pattern during oogenesis of zebrafish was examined using in situ hybridization and fluorescent quantitative RT-PCR. During zebrafish oogensis, vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage II oocytes, followed by a distribution among vacuome in stage III. Later in stage IV and V, vasa mRNA is enriched at the cortex and finally localized at the cortex. The fluorescent quantitative RT-PCR shows that the quantity of vasa mRNA decreases from stage II to stage III, but remains relatively invariable from stage III to stage V. The observed differences in vasa mRNA expression in the different stages of zebrafish oogenesis suggest that vasa gene plays an important role during oogenesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index