| Autor: |
Yang YANG, Qian SHI, Ze LIU, Ruo-jie LI, Peng-wei PAN, Yuan-yuan HOU, Wan-ge LU, Gang BAI |
| Předmět: |
|
| Zdroj: |
Acta Pharmacologica Sinica; Apr2010, Vol. 31 Issue 4, p443-449, 7p, 1 Chart, 6 Graphs |
| Abstrakt: |
AbstractAim:To investigate the efficacy of glycyrrhizin (GL) combined with salbutamol (SA) as an anti-asthma therapy.Methods:Rat lung beta2-adrenergic receptor (β2-AR) mRNA level was measured by real-time RT PCR. Intracellular cAMP accumulation was evaluated with a reporter gene assay. An in vitro acetylcholine-induced guinea pig tracheal strip contraction model was used to test the relaxing effects of GL and SA. The anti-inflammatory effects of GL and SA were tested using tumor necrosis factor-α-induced NF-κB transcriptional activation reporter assay, I-κB Western blotting and interleukin-8 ELISA. An in vivo guinea pig asthma model was used to prove further the synergistic effect of GL and SA.Results:GL (0.3 μmol/L) increased mRNA levels of β2-AR in vivo and the accumulation of cAMP in vitro. The combination of GL and SA also resulted in significant complementary anti-inflammatory effects via inhibition of NF-κB activation, degradation of I-κB and production of interleukin-8. A significant synergistic effect of the combination was detected both in vitro and in vivo in a guinea pig mode.Conclusion:The results demonstrate that GL and SA have synergistic anti-asthmatic effects and offer the possibility of a therapeutic application of GL in combination with β2-AR agonists in the treatment of asthma. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
