| Autor: |
Dmitry I. Strokotov, Maxim A. Yurkin, Konstantin V. Gilev, Dirk R. van Bockstaele, Alfons G. Hoekstra, Nikolay B. Rubtsov, Valeri P. Maltsev |
| Předmět: |
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| Zdroj: |
Journal of Biomedical Optics; Nov2009, Vol. 14 Issue 6, p064036-064036-12, 1p |
| Abstrakt: |
We characterize T- and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T- and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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