| Autor: |
Chardonnens, D., Cameo, P., Aubert, M.L., Pralong, F.P., Islami, D., Campana, A., Gaillard, R.C., Bischof, P. |
| Zdroj: |
Molecular Human Reproduction; Nov99, Vol. 5 Issue 11, p1077, 6p |
| Abstrakt: |
To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17β-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17β-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean ± SEM). Basal CTB leptin production was 25.2 ± 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 ± 16% (P < 0.0001) and 195 ± 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1α respectively. 17β-oestradiol 10to 10mol/l increased basal leptin production 5-9-fold, while 10mol/l had no such effect. Basal CTB HCG secretion was 5722 ± 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 μg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5x10mol/l significantly increased HCG secretion to 140 ± 21% of controls (P = 0.031). Cetrorelix (0.1 μg/ml) inhibited leptin-induced HCG secretion (P = 0.0028). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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