| Autor: |
Berenyi, M., Mauleon, R. P., Kopecky, D., Waldl, S., Friedl, R., Fluch, S., Boonruangrod, R., Muge, E., Burg, K. |
| Předmět: |
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| Zdroj: |
Plant Molecular Biology Reporter; Mar2009, Vol. 27 Issue 1, p79-85, 7p, 2 Charts, 1 Graph |
| Abstrakt: |
For the isolation of gene space representative sequence elements, a new methodology—high C+G patch (HCGP) filtration—has been developed using rice as a model. The method is based on the fragmentation of the genomic DNA by methylation-sensitive HpaII and MspI restriction endonucleases having exclusively G/C base pair-containing recognition sites. These enzymes fragment the genome at high C+G content and hypomethylated regions. Cloning fragments spanning such regions in close vicinity (200–2,000 bp) revealed that about 60% of the clones represented gene space sequences resulting in twofold enrichment of these sequences, which is close to the theoretical maximum in rice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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