| Autor: |
Seymen, Hakki Oktay, Seymen, Pinar, Aytac, Erman, Demir, Fatih, Bolukbasi, Feray, Saygili, Seha, Ozucer, Berke, Dikmen, Goksel, Genc, Habibe, Altug, Tuncay, Uzun, Hafize |
| Předmět: |
|
| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA1278-A1278, 1/4p |
| Abstrakt: |
Neuron-specific enolase(NSE) is a dimeric isoenzyme of the glycolytic enzyme enolase. S100 beta is a small, acidic, calcium binding protein which is highest in concentration in the vertebrate nervous system. S-100B is found in high concentrations in astroglial and Schwann cells. 44 Wistar-albino adult rats were divided into four groups: Saline-treated group(S) (n=10), Saline-Darbepoetin-treated group (SD)(n=10), Ethanol- treated group (ET) (n=12), Ethanol-Darbepoetin-treated group (ETD) (n=12). 20% ethanol solution prepared with sterile saline was administered to rats ip at a dosage of 2.5 g/kg for two times with a 2-h interval. Darbepoetin was administered ip just after the second dose of ethanol at a dosage of 10 µg/kg. Twenty-four hours after the first dose of ethanol, the animals were decapitated under Ketamine (5-10ml/kg). In this study, the effect of darbepoetin administration in ethanol toxicity on brain tissue's oxidant-antioxidant parameters which are Malonyl dialdehyde(MDA), Cu-Zn Superoxide dismutase(Cu-Zn SOD), Glutathione(GSH) and Catalase(CAT); neuronal damage markers which are S-100Beta (CanAg SI00 EIA REF 708-10, Sweden) and Netiron Specific Enolase(NSE) (CanAg NSE EIA REF 420-10) was evaluated. Ethanol increases significantly SI00 levels(p<0.05) in ET. Darbepoetin administration decreases significantly NSE levels in ETD(p<0.01). Darbepoetin might be protect against ethanol induced neurodegenerative effect. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text