| Autor: |
Chun-Shiang Chung, Watkins, Lara, Funches, Antonio, Lonias-Neira, Joanne, Cioffi, William G., Ayala, Alfred |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Regulatory, Integrative & Comparative Physiology; Nov2006, Vol. 291, pR1338-R1343, 6p, 5 Graphs |
| Abstrakt: |
Studies have indicated that γδ T lymphocytes play an important role in the regulation of immune function and the clearance of intracellular pathogens. We have recently reported that intraepithelial lymphocytes (IEL). which are rich in γδ T cells, within the small intestine illustrated a significant increase in apoptosis and immune dysfunction in mice subjected to sepsis. However, the contribution of γδ T cells to the host response to polymicrobial sepsis remains unclear. In this study. we initially observed that after sepsis induced by cecal ligation and puncture (CLP). there was an increase in small intestinal IEL CD8+ γδ+ T cells in control γδ+/+ mice. Importantly, we subsequently found an increased early mortality in mice lacking γδ T cells (γδ-/- mice) alter sepsis. This was associated with decreases in plasma TNF-α. lL-6, and IL-12 levels in γδ-/- mice compared with γδ+/+ mice after sepsis. In addition, even though in vitro LPS-stimulated peritoneal macrophages showed a reduction in IL-6 and IL-12 release after CLP, these cytokines were less suppressed in macrophages isolated from γδ-/- mice. Alternatively, IL-10 release was not different between septicγδ+/+ and γδ-/- mice. Whereas T helper (Th)1 cytokine release by anti-CD3-stimulated splenocytes was significantly depressed in septic γδ+/+ mice, there was no such depression in γδ-/- mice. However, γδ T cell deficiency had no effect on Th2 cytokine release. These findings suggest that γδ T cells may play a critical role in regulating the host immune response and survival to sepsis, in part by alteration of the level of IEL CD8+γδ+ T cells and through the development of the Th1 response. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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