| Abstrakt: |
Studies on Gulf War veterans with depleted uranium (DU) fragmentsembedded in their soft tissues have led to suggestions of possible DUinduced neurotoxicity. We investigated DU uptake into cultured rat brainendothelial cells (RBE4). Following the determination that DU readilyenters RBE4 cells, cytotoxic effects were analyzed using assays for cell volumeincrease, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol- 2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactatedehydrogenase (LDH) activity. The results of these studies show that uptake of the U3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm israther reassuring and provides no indication for overt cytotoxicity inendothelial cells exposed to DU. [ABSTRACT FROM AUTHOR] |
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