Characteristics of rat bone marrow cells differentiated into a liver cell lineage and dynamics of the transplanted cells in the injured liver.

Autor: Okumoto, Kazuo, Saito, Takafumi, Haga, Hiroaki, Hattori, Etsuko, Ishii, Rika, Karasawa, Tetsuru, Suzuki, Akihiko, Misawa, Keiko, Sanjo, Mai, Ito, Jun-itsu, Sugahara, Kazuhiko, Saito, Koji, Togashi, Hitoshi, Kawata, Sumio
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Zdroj: Journal of Gastroenterology; Jan2006, Vol. 41 Issue 1, p62-69, 8p, 4 Diagrams, 3 Cartoon or Caricatures
Abstrakt: Background. Bone marrow cells (BMCs) have been shown to differentiate into a liver cell lineage, but little is known about their dynamics following transplantation. BMCs were cultured to investigate the expression of liver-specific genes in vitro and transplanted into in vivo liver-injury models to elucidate their dynamics in the liver. Methods. The mRNA expression of various liver-specific genes in BMCs cocultured with hepatocytes was analyzed using reverse transcription-polymerase chain reaction. BMCs from transgenic rats expressing green fluorescent protein were transplanted into the spleen of rat liver-injury models induced with 2-acetylaminofluorene (2-AAF) or carbon tetrachloride (CCl4). BMCs were also transplanted directly into livers treated with CCl4 to determine which route is better for transplantation. Results. BMCs differentiated into a liver cell lineage in vitro and expressed mRNAs consistent with mature hepatocytes, including albumin. The transplanted BMCs were found in the liver in the CCl4-induced injury model, but not in the 2-AAF-induced model. The hepatocyte growth factor and fibroblast growth factor mRNA levels in the liver were significantly higher in the CCl4-induced model than in the 2-AAF-induced model. Migration of BMCs to the liver was more effective following injection into the liver, rather than into the spleen. Conclusions. Cultured BMCs differentiated into a liver cell lineage are a potential source for cell transplantation. Transplantation is successful in the severely injured liver with a high level of expression of mRNAs for growth factors. Injection of BMCs directly into the liver is the preferred route of administration. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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