Biological Synthesis of Silver Nanoparticles Using Klebsiella pneumoniae (ON640793) and Assessment of Its Antibacterial, Larvicidal and Biotoxicity Efficiency.
Autor: | Wilson, Jeyaraj John, Mahalakshmi, S., Thangaraj, Ramasamy, Mahendran, Shunmugiah, Sivakumar, Thangavel, Sudarmani, D. N. P., Immanuvel, A., Venkidasamy, Baskar |
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Zdroj: | BioNanoScience; Mar2025, Vol. 15 Issue 1, p1-15, 15p |
Abstrakt: | This study aimed to determine the effectiveness of bacteria-synthesized silver nanoparticles (AgNPs) in larvicidal and biotoxicity assay. Klebsiella pneumoniae (ON640793) bacteria was obtained from a soil sample collected from the Western Ghats. The extracellular synthesized AgNPs from K. pneumoniae were examined by distinct analyses, such as Ultraviolet–visible (UV–vis) spectrophotometer, Fourier transform infrared (FTIR) spectrometer, particle size analysis, atomic force microscopy (AFM), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and X-ray diffraction (XRD). The antibacterial efficacy of AgNPs produced by K. pneumoniae was examined against human pathogenic microorganisms such as Proteus mirabilis, Escherichia coli, Serratia sp., Pseudomonas aeruginosa, and Staphylococcus aureus was determined using disc diffusion. At 40 µg/mL concentrations, the highest zone of inhibition for AgNPs was noticed in Proteus mirabilis (1.73 ± 0.43 cm), followed by, Serratia (1.13 ± 0.26 cm), Escherichia coli (1.5 ± 0.3 cm), Pseudomonas aeruginosa (0.9 ± 0.173 cm), and Staphylococcus aureus (0.83 ± 0.26 cm). The synthesized K. pneumoniae (ON640793) AgNPs exhibited strong larvicidal activity against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus fourth instar larvae. Greater mortality was recorded, with LC |
Databáze: | Complementary Index |
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