| Abstrakt: |
Background: Type 2 diabetes (T2D) has already become a global pandemic. As its simple, rapid, economical, and relatively non-invasive, metabolic markers have become a method for T2D diagnosis. However, region, race, and diet all affect the metabolism of the body. The purpose of current study is to explore the differences of metabolites in T2D patients from regions. Methods: We recruited 103 T2D patients in two clinical centers, including 52 T2D patients from Beijing (T2DB) and 51 T2D patients from Kaifeng (T2DK). The serum samples from T2D patients were analyzed using high-resolution mass spectrometer. After screened using univariate and multivariate analysis, the differential metabolites were identified. Moreover, to reveal biological information, we performed pathway analysis with the differential metabolites. Results: Thirty-six differential metabolites were identified, including 16 metabolites were higher concentrations while 20 metabolites were lower concentrations in the serum of T2DB patients than T2DK patients. There were higher serum concentrations of L-phenylalanine, 4-methyl-2-oxovaleric acid, L-carnitine, decanoylcarnitine, 9-decenoylcarnitine and sphinganine in T2DB patients, in which decanoylcarnitine in T2DB patients was up to 35-fold higher than T2DK patients. While there were lower concentrations of L-valine, L-isoleucine, arachidonic acid, oleic acid, 16-hydroxyhexadecanoic acid, lysophosphatidylcholine (18:0) and 1-Phenylethylamine in T2DB patients, in which 1-phenylethylamine in T2DB patients was decreased to 0.45-fold lower than T2DK patients. The reason for the differences might be that phosphatidylethanolamine biosynthesis, phosphatidylcholine biosynthesis, valine, leucine and isoleucine degradation, and beta-oxidation of very long chain fatty acids were different in T2DB patients and in T2DK patients. Conclusion: Metabolites from different pathways are independently related to regions, providing valuable insight and potential for the diagnosis and treatment of T2D. [ABSTRACT FROM AUTHOR] |
