| Abstrakt: |
The limitations of the usual and traditional taxonomy have caused a large number of arthropods, especially insects, have not yet been identified. In recent years, DNA barcoding, which is based on the variation of a short sequence of DNA, has provided a new alternative method for species identification. This method is innovative, fast, accurate, reliable, and applicable to a wide range of multicellular animals including insects. The DNA barcoding method is an important branch of biodiversity science that fills the gap between molecular and traditional methods for species identification. This method has provided a suitable framework for identifying many unknown species, important species, and hidden species. In addition, it has provided the basis for the identification of different species of insects based on their immature stages (including eggs, larvae, and nymphs) that cannot be identified by traditional methods. With all these positive points in using the barcode method, this method suffers from some limitations. Issues such as speciation, hybridization, and excessive contamination of insects with symbiotic species such as Wolbachia bacteria, which make the results of this method problematic, cause limitations for this method. Most importantly, the reliability of the mentioned method has been questioned considering that more than 1 million insects have been identified and millions of other species have not yet been identified. This high amount of diversity of insect species has caused the amount of data obtained from the barcoding method cannot respond to the high biodiversity of insects. According to the stated contents, it seems that to identify the species, a combination of molecular methods such as barcoding and traditional methods should be used to identify living animals, especially insects. [ABSTRACT FROM AUTHOR] |
