| Autor: |
Rahmani, Milad, Pourmadadi, Mehrab, Shakouri, Sayeh, Rahdar, Abbas, Díez-Pascual, Ana M. |
| Zdroj: |
BioNanoScience; Dec2024, Vol. 14 Issue 5, p5286-5296, 11p |
| Abstrakt: |
Due to the increasing global incidence of cancer and the undesirable side effects of conventional therapies, developing drug delivery systems based on nanomaterials is crucial. In this research, a novel and pH-responsive nanocomposite composed of gelatin/polyvinyl alcohol/titanium dioxide (G/PVA/TiO2) was fabricated through water-in-oil-in-water (W/O/W) emulsification and applied for the delivery of the quercetin (QC) drug. The G/PVA/TiO2/QC drug delivery system exhibited 45.7% drug loading and 88.25% encapsulation efficiency due to a high interpenetrating biopolymeric network and the porous structure of TiO2. These values are competitive and higher than those reported for other QC drug delivery systems. FT-IR and XRD analyses confirmed the successful synthesis of the nanocomposite and the crystalline nature of its components. FESEM and zeta potential analyses were conducted to examine the morphology and surface charge, respectively. The zeta potential value of 23.6 mV confirmed the good stability of the nanocomposite. Experiments using the dialysis technique revealed higher amount of QC released in a lower pH environment, mimicking a tumor environment, compared to neutral pH, confirming the pH-sensitivity of the G/PVA/TiO2/QC nanocomposite. Additionally, it exhibited significant cytotoxicity against MCF-7 cells compared to free QC, as indicated by MTT test results. The flow cytometry assay confirmed that G/PVA/TiO2/QC led to a higher apoptotic percentage than free QC, while the free drug showed a higher percentage of necroptotic cells. Schematic illustration depicting the behavior of the QC-loaded nanocomposite at different pHs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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