| Abstrakt: |
This study aimed to assess the growth and development of 42 kDa chitinase-transgenic peanut lines in the T1 progeny and their phytopathogenic fungus resistance. Line S1A-12 contains the syncodChi42-1 gene, line S2A-11 contains the syncodChi42-2 gene, and lines WTA-3 and WTA-5 contain the Chi42 gene. The Chi42 gene is derived from Trichoderma asperellum SH16. SyncodChi42-1 and syncodChi42-2 are sequences of the Chi42 gene optimized for codon usage in plant expression. The study results showed that all four transgenic peanut lines maintained fungal resistance in T1 progeny. However, only three lines, S1A-12, WTA-3, and WTA-5, were segregated in the Mendelian ratio (3:1). T1 transgenic peanut lines grew more vigorously and produced higher numbers of mature pods, 100-pod weight, and 100-seed weight than the non-transgenic control. In which the S2A-11 line exhibited the strongest growth and development ability. The four transgenic lines had a higher protein content than the control, but their lipid and reducing sugar contents were similar. These findings suggest that transgenic peanuts containing one of two optimized chitinase genes may be promising candidates for the production of peanut cultivars resistant to phytopathogenic fungi. [ABSTRACT FROM AUTHOR] |
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