Proliferation associated 2G4 is required for the ciliation of vertebrate motile cilia.

Autor: Lee, Moonsup, Carpenter, Christina, Hwang, Yoo-Seok, Yoon, Jaeho, Lu, Quanlong, Westlake, Christopher J., Moody, Sally A., Yamaguchi, Terry P., Daar, Ira O.
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Zdroj: Communications Biology; 11/4/2024, Vol. 7 Issue 1, p1-11, 11p
Abstrakt: Motile cilia are critical structures that regulate early embryonic development and tissue homeostasis through synchronized ciliary motility. The formation of motile cilia is dependent on precisely controlled sequential processes including the generation, migration, and docking of centrioles/basal bodies as well as ciliary growth. Using the published proteomics data from various organisms, we identified proliferation-associated 2G4 as a novel regulator of ciliogenesis. Loss-of-function studies using Xenopus laevis as a model system reveal that Pa2G4 is essential for proper ciliogenesis and synchronized movement of cilia in multiciliated cells (MCCs) and the gastrocoel roof plate (GRP). Pa2G4 morphant MCCs exhibit defective basal body docking to the surface as a result of compromised Rac1 activity, apical actin network formation, and immature distal appendage generation. Interestingly, the regions that include the RNA-binding domain and the C-terminus of Pa2G4 are necessary for ciliogenesis in both MCCs and GRP cells. Our findings may provide insights into motile cilia-related genetic diseases such as Primary Ciliary Dyskinesia. The study on the role of Proliferation-associated 2G4 in motile cilia formation and synchronized cilia movement offers valuable insights into research on motile cilia-related genetic diseases. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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