| Autor: |
Janssen, Aafke W. F., van der Lugt, Benthe, Duivenvoorde, Loes P. M., Vos, Arjan Paul, Bastiaan-Net, Shanna, Tomassen, Monic M. M., Verbokkem, Janine A. C., Blok-Heimerikx, Emmie, Hooiveld, Guido J. E. J., van Baarlen, Peter, Ferrier, Laurent, van der Zande, Meike |
| Předmět: |
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| Zdroj: |
Scientific Reports; 11/2/2024, Vol. 14 Issue 1, p1-14, 14p |
| Abstrakt: |
To investigate intestinal health and its potential disruptors in vitro, representative models are required. Human induced pluripotent stem cell (hiPSC)-derived intestinal epithelial cells (IECs) more closely resemble the in vivo intestinal tissue than conventional in vitro models like human colonic adenocarcinoma Caco-2 cells. However, the potential of IECs to study immune-related responses upon external stimuli has not been investigated in detail yet. The aim of the current study was to evaluate immune-related effects of IECs by challenging them with a pro-inflammatory cytokine cocktail. Subsequently, the effects of Lactiplantibacillus plantarum WCFS1 were investigated in unchallenged and challenged IECs. All exposures were compared to Caco-2 cells and in vivo data where possible. Upon the inflammatory challenge, IECs and Caco-2 cells induced a pro-inflammatory response which was strongest in IECs. Heat-killed L. plantarum exerted the strongest effect on immune parameters in the IEC model, while L. plantarum in the stationary growth phase had most pronounced effects on immune-related gene expression in Caco-2 cells. Unfortunately, comparison to in vivo transcriptomics data showed limited similarities, which could be explained by essential differences in the study setups. Altogether, hiPSC-derived IECs show a high potential as a model to study immune-related responses in the intestinal epithelium in vitro. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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