| Abstrakt: |
Kampong chickens are produced widely throughout the Indonesian provinces, such as East Nusa Tenggara, especially in East Manggarai Regency. Raising kampong chickens on a microscale is mostly intended to meet the protein needs of meat and eggs. However, the production of kampong chickens has decreased due to environmental factors and pathogenic bacteria that cause infectious diseases. The disease is usually treated with antibiotics. Intensive application of antibiotics causes pathogen resistance to these antibiotics. A good solution to eco-friendly and sustainable kampong chicken raising is probiotic application. Probiotic bacteria generally come from the lactic acid bacteria (LAB) group. In previous studies, lactic acid bacteria with probiotic properties were found in the cecum of Kampong chickens, such as isolates AK1-15, AK1-19, and AK1-22. This study aimed to identify LAB with probiotic properties and determine the susceptibility of LAB to antibiotics. An antibiotic resistance test was carried out using the paper disc diffusion method. Identification of probiotic bacteria isolates used procedures according to the NEXprepTM DNA kit and sequencing based on 16S rDNA sequences. Data were analyzed using a two-way ANOVA. The results showed that isolates AK1 15, AK1 19, and AK1 22 were resistant to the antibiotics chloramphenicol, cefadroxil, doxycycline, tetracycline, spiramycin, erythromycin, amoxicillin, ampicillin, and rifampicin. Isolate AK1 15 was identified as Enterococcus faecium NBRC 100486 with 99.9% similarity, while AK1 19 and AK1 22 were identified as Enterococcus faecium P12-2 with 99.8% and 99.9% similarity, respectively. These probiotic bacteria can be applied to kampong chickens. Probiotics can be mixed into chicken feed to improve the health of kampong chickens. [ABSTRACT FROM AUTHOR] |
