Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics.

Autor: Zhu, Guijie, Sadeghi, Seyed Amirhossein, Mahmoudi, Morteza, Sun, Liangliang
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Zdroj: Chemical Communications; 10/18/2024, Vol. 60 Issue 81, p11528-11531, 4p
Abstrakt: The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index