| Autor: |
Martínez Mir, Clara, Pisterzi, Paola, De Poorter, Isabel, Rilou, Maria, van Kranenburg, Melissa, Heijs, Bram, Alemany, Anna, Sage, Fanny, Geijsen, Niels |
| Předmět: |
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| Zdroj: |
Communications Biology; 10/6/2024, Vol. 7 Issue 1, p1-16, 16p |
| Abstrakt: |
Myofibers are large multinucleated cells that have long thought to have a rather simple organization. Single-nucleus transcriptomics, spatial transcriptomics and spatial metabolomics analysis have revealed distinct transcription profiles in myonuclei related to myofiber type. However, the use of local tissue collection or dissociation methods have obscured the spatial organization. To elucidate the full tissue architecture, we combine two spatial omics, RNA tomography and mass spectrometry imaging. This enables us to map the spatial transcriptomic, metabolomic and lipidomic organization of the whole murine tibialis anterior muscle. Our findings on heterogeneity in fiber type proportions are validated with multiplexed immunofluorescence staining in tibialis anterior, extensor digitorum longus and soleus. Our results demonstrate unexpectedly strong regionalization of gene expression, metabolic differences and variable myofiber type proportion along the proximal-distal axis. These new insights in whole-tissue level organization reconcile sometimes conflicting results coming from previous studies relying on local sampling methods. Multi-dimensional analysis of gene expression, myofiber types and metabolic activity in whole skeletal muscle reveals specific regionalization of gene expression and metabolites related to the glycolytic and oxidative potential of myofibers [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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