| Autor: |
Gláucia-Silva, Fiamma, Torres, João Vicente Pereira, Torres-Rêgo, Manoela, Daniele-Silva, Alessandra, Furtado, Allanny Alves, Ferreira, Sarah de Sousa, Chaves, Guilherme Maranhão, Xavier-Júnior, Francisco Humberto, Rocha Soares, Karla Samara, Silva-Júnior, Arnóbio Antônio da, Fernandes-Pedrosa, Matheus de Freitas |
| Předmět: |
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| Zdroj: |
International Journal of Molecular Sciences; Sep2024, Vol. 25 Issue 18, p9893, 14p |
| Abstrakt: |
The rapid resistance developed by pathogenic microorganisms against the current antimicrobial pool represents a serious global public health problem, leading to the search for new antibiotic agents. The scorpion Tityus stigmurus, an abundant species in Northeastern Brazil, presents a rich arsenal of bioactive molecules in its venom, with high potential for biotechnological applications. However, venom cytotoxicity constitutes a barrier to the therapeutic application of its different components. The objective of this study was to produce T. stigmurus-venom-loaded cross-linked chitosan nanoparticles (Tsv/CN) at concentrations of 0.5% and 1.0% to improve their biological antimicrobial activity. Polymeric nanoparticles were formed with a homogeneous particle size and spherical shape. Experimental formulation parameters were verified in relation to mean size (<180 nm), zeta potential, polydispersity index and encapsulation efficiency (>78%). Tsv/CN 1.0% demonstrated an ability to increase the antimicrobial venom effect against Staphylococcus aureus bacteria, exhibiting an MIC value of 44.6 μg/mL. It also inhibited different yeast species of the Candida genus, and Tsv/CN 0.5% and 1.0% led to a greater inhibitory effect of C. tropicalis and C. parapsilosis strains, presenting MIC values between 22.2 and 5.5 µg/mL, respectively. These data demonstrate the biotechnological potential of these nanosystems to obtain a new therapeutic agent with potential antimicrobial activity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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