| Abstrakt: |
Biotic stress leads to enormous yield, quality, and economic losses in grapevine (Vitis vinifera L.). However, the crucial genes and underlying mechanisms in grapevine response to biotic stress are still not fully understood. Therefore, a meta-analysis was done using microarray expression data from 114 samples from five biotic stress investigations to find differentially expressed genes (DEGs). Subsequently, 1005 DEGs yielded from the meta-analysis were subjected to further analysis using a combination of systems biology and machine learning algorithms. A total of 75 transcription factors (TFs) and 80 pathogen recognition genes (PRGs) were identified among the 1005 DEGs. The majority of upregulated PRGs belonged to a class of kinases (KIN), followed by the RLK, RLP, TRAN, N, and CK classes. Furthermore, the MYB and bHLH families had the highest number of up-regulated TFs in response to biotic stress. Their co-expression with biotic stress-related pathways highlights the crucial role of these families in the response to biotic stress in grapevine. Additionally, weighted gene co-expression network analysis identified six modules, three of which were upregulated and relevant to defense responses, photosynthesis process, and cell wall reinforcement. Using eight attribute weighting algorithms on the identified key genes, we prioritized eight genes with the highest relevance to both control and disease conditions. Our findings indicate that the transcriptome responses of grapevine plants to various biotic stimuli, including downy mildew, powdery mildew, grapevine leaf-roll-associated virus-3, and Bois noir phytoplasma, exhibit commonalities. These shared responses could serve as breeding targets for enhancing grapevine resistance to diverse biotic stresses. [ABSTRACT FROM AUTHOR] |
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