Discovery of a new bacterium, Microbacterium betulae sp. nov., in birch wood associated with hypersensitivity pneumonitis in woodworkers.

Autor: Paściak, Mariola, Pawlik, Krzysztof J., Martynowski, Dariusz, Łaczmański, Łukasz, Ciekot, Jarosław, Szponar, Bogumiła, Wójcik‐Fatla, Angelina, Mackiewicz, Barbara, Farian, Ewelina, Cholewa, Grażyna, Cholewa, Alicja, Dutkiewicz, Jacek
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Zdroj: Environmental Microbiology Reports; Aug2024, Vol. 16 Issue 4, p1-15, 15p
Abstrakt: A Gram‐positive, aerobic, rod‐shaped mesophilic bacterium was isolated from birch wood, referred to as the AB strain. Allergological tests suggest that this strain may cause allergic alveolitis in sawmill workers. Employing a polyphasic taxonomic approach, the AB strain's 16S rRNA gene sequence showed high similarity to Microbacterium barkeri and M. oryzae, with 97.25% and 96.91%, respectively, a finding supported by rpoB and gyrB sequence analysis. Further genome sequence comparison with the closely related M. barkeri type strain indicated a digital DNA–DNA hybridization value of 25.5% and an average nucleotide identity of 82.52%. The AB strain's cell wall peptidoglycan contains ornithine, and its polar lipids comprise diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Its major fatty acids include anteiso C15:0, anteiso C17:0, and iso C16:0, while MK‐10 is its predominant respiratory quinone. Comprehensive analysis through 16S rRNA, whole‐genome sequencing, phenotyping, chemotaxonomy, and MALDI‐TOF MS profiling indicates that the AB strain represents a new species within the Microbacterium genus. It has been proposed to name this species Microbacterium betulae sp. nov., with ABT (PCM 3040T = CEST 30706T) designated as the type strain. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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