Dioscin attenuates lupus nephritis in NZB/W F1 mice by decreasing NF-κB activation and NLRP3 inflammasome.
| Autor: | Yaling Xu, Han Li |
|---|---|
| Předmět: |
NF-kappa B
PROTEINURIA LUPUS nephritis CREATININE RESEARCH funding IMMUNOGLOBULINS TREATMENT effectiveness PREDNISONE BLOOD urea nitrogen FLUORESCENT antibody technique CELLULAR signal transduction MICE INTERFERONS GENE expression GLYCOSIDES ANIMAL experimentation WESTERN immunoblotting OSTEOARTHRITIS CARTILAGE cells SIGNAL peptides KIDNEYS INTERLEUKINS TUMOR necrosis factors GLOMERULAR filtration rate |
| Zdroj: | Folia Histochemica et Cytobiologica; 2024, Vol. 62 Issue 2, p110-121, 12p |
| Abstrakt: | Introduction. Dioscin, a natural steroid saponin, has anticancer, anti-inflammatory, anti-hyperlipidemic, and glycemic capabilities. This study focused on dioscin roles and its related mechanisms in experimental lupus nephritis. Materials and methods. Lupus-prone NZB/W F1 mice were intragastrically administered with dioscin, prednisone or vehicle, and kidney, urine and blood samples were harvested after the mice were sacrificed. Proteinuria, blood urea nitrogen (BUN), creatinine, anti-dsDNA, IL-1β, and IL-18 levels in serum as well as IFN-γ, IL-6, IL-17 and TNF-α levels in kidney tissues were assessed. Renal histopathology was examined through hematoxylin-eosin staining. IgG and C3 expression in kidney was evaluated using immunofluorescence staining. The number of glomerular F4/80-positive cells and NLRP3-positive cells was determined by immunohistochemical staining. The protein expression was examined by western blotting. Results. Dioscin alleviated lupus nephritis in NZB/W F1 mice. Dioscin declined serum anti-dsDNA level, prevented deposition of immune complexes in renal glomeruli, and inhibited the inflammatory response and infiltration of macrophages into mouse kidneys. Dioscin inhibited NF-κB and NLRP3 inflammasome in NZB/W F1 mice. Conclusions. Dioscin ameliorates lupus nephritis through inhibition of NLRP3 inflammasome and NF-κB signaling. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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