The Assessment of Sperm DNA Integrity: Implications for Assisted Reproductive Technology Fertility Outcomes across Livestock Species.

Autor: Robertson, Maya J., Chambers, Caitlin, Spanner, Eloise A., de Graaf, Simon P., Rickard, Jessica P.
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Zdroj: Biology (2079-7737); Jul2024, Vol. 13 Issue 7, p539, 22p
Abstrakt: Simple Summary: This review explores the importance of sperm DNA integrity in assisted reproductive technologies used in both livestock production and human fertility treatments. Assisted reproductive technologies have revolutionised animal breeding by enabling the widespread dissemination of elite male genetics and overcoming human infertility challenges, with male-factor infertility being a significant concern. Traditional semen quality assessments focus on parameters like volume, concentration, motility and morphology; however, DNA integrity, a potential key parameter in assessing semen quality, is not included despite being studied for many years. Various assays are used to measure sperm DNA fragmentation, but results on its impact on fertility are inconsistent, resulting in a lack of standardisation of assessment methodology. This review consolidates findings across species and DNA integrity assays into a comprehensive table, highlighting DNA integrity as a potential biomarker for male infertility and predictive value for assisted reproductive technology outcomes in livestock and humans. Sperm DNA integrity is increasingly considered a useful measure of semen quality in mammalian reproduction. However, the definition of DNA integrity, the ideal means by which it should be measured, and its predictive value for fertility remain a topic of much discussion. With an emphasis on livestock species, this review discusses the assays that have been developed to measure DNA integrity as well as their correlation with in vitro and in vivo fertility. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index