| Autor: |
Gao, Tian, Wang, Qiong, Sun, Huarui, Liu, Yang, Li, Jitao, He, Yuying |
| Předmět: |
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| Zdroj: |
Biology (2079-7737); Jul2024, Vol. 13 Issue 7, p488, 15p |
| Abstrakt: |
Simple Summary: High carbonate alkalinity and high pH are the main characteristics of saline–alkaline water environments. This study aimed to elucidate the physiological mechanism of the hepatopancreas of Fenneropenaeus chinensis in response to saline–alkaline stress. Proteomics and metabolomics of hepatopancreas were employed to analyze the effects of high carbonate alkalinity (CA) stress and combined high carbonate alkalinity and high pH (CP) stress on shrimps after 24 h. The results indicated that several key proteins and metabolites involved in carbohydrate metabolism and fatty acid oxidation were significantly upregulated. Additionally, the antioxidant and immune systems were found to have been affected. These findings suggest that CA and CP stressors induced oxidative stress in the hepatopancreas of F. chinensis, resulting in impaired immunity and diminished inflammatory responses. F. chinensis may resist osmotic pressure imbalances caused by CA or CP stress by upregulating the energy metabolism level in the hepatopancreas. Our findings may offer valuable insights for further investigations into the saline–alkaline water culture of F. chinensis. The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize the abundant inland saline–alkaline water, we studied the physiological effects of high carbonate alkalinity stress and high pH stress on Fenneropenaeus chinensis. The study employed quantitative proteomics by tandem mass tag (TMT) and non-targeted metabolomics analysis using a liquid chromatograph mass spectrometer (LC-MS) to understand the physiological and biochemical adaptive mechanisms of the hepatopancreas of F. chinensis in response to saline–alkaline stress at the molecular level. We designed two stress groups as follows: a high carbonate alkalinity (CA) group and a combined high carbonate alkalinity and high pH (CP) group. The study found that the protein and metabolic profiles of the two stress groups were changed, and the CP group, which was exposed to dual stresses, incurred more severe damage to the hepatopancreas compared to that of the CA group. After exposure to CA and CP, the hepatopancreas of F. chinensis showed significant alterations in 455 proteins and 50 metabolites, and 1988 proteins and 272 metabolites, respectively. In addition, F. chinensis upregulated the level of energy metabolism in the hepatopancreas to defend against osmotic imbalance caused by CA or CP stress, which was demonstrated by the significant upregulation of important proteins and metabolites in glycolysis, pyruvate metabolism, TCA cycle, and fatty acid oxidation. Additionally, pattern recognition receptors, the phenol oxidase system, and various immune-related metabolic enzymes and metabolites were also affected. The immune homeostasis of F. chinensis was affected by the alteration of the antioxidant system following exposure to CA or CP. These findings provide valuable information for F. chinensis saline–alkaline water cultivation practices. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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