Maize auxin response factor ZmARF1 confers multiple abiotic stresses resistances in transgenic Arabidopsis.

Autor: Liu, Ling, Gong, Ying, Yahaya, Baba Salifu, Chen, Yushu, Shi, Dengke, Liu, Fangyuan, Gou, Junlin, Zhou, Zhanmei, Lu, Yanli, Wu, Fengkai
Zdroj: Plant Molecular Biology; Aug2024, Vol. 114 Issue 4, p1-16, 16p
Abstrakt: Prolonged exposure to abiotic stresses causes oxidative stress, which affects plant development and survival. In this research, the overexpression of ZmARF1 improved tolerance to low Pi, drought and salinity stresses. The transgenic plants manifested tolerance to low Pi by their superior root phenotypic traits: root length, root tips, root surface area, and root volume, compared to wide-type (WT) plants. Moreover, the transgenic plants exhibited higher root and leaf Pi content and upregulated the high affinity Pi transporters PHT1;2 and phosphorus starvation inducing (PSI) genes PHO2 and PHR1 under low Pi conditions. Transgenic Arabidopsis displayed tolerance to drought and salt stress by maintaining higher chlorophyll content and chlorophyll fluorescence, lower water loss rates, and ion leakage, which contributed to the survival of overexpression lines compared to the WT. Transcriptome profiling identified a peroxidase gene, POX, whose transcript was upregulated by these abiotic stresses. Furthermore, we confirmed that ZmARF1 bound to the auxin response element (AuxRE) in the promoter of POX and enhanced its transcription to mediate tolerance to oxidative stress imposed by low Pi, drought and salt stress in the transgenic seedlings. These results demonstrate that ZmARF1 has significant potential for improving the tolerance of crops to multiple abiotic stresses. Key message: ZmARF1 is a pleiotropic gene which confers tolerance to multiple abiotic stresses in ZmARF1-OE transgenic Arabidopsis as revealed by stress-response phenotypes of the transgenic lines relative to WT. ZmARF1 confers stress tolerance through a mechanism involving its association with an antioxidant enzyme, AtPOX, whose expression was simultaneously up regulated by low Pi, drought and salt stresses, whiles its promoter activity was significantly activated by ZmARF1. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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