Parallel detection of multiple biomarkers in a point-of-care-competent device for the prediction of exacerbations in chronic inflammatory lung disease.

Autor: Röckendorf, Niels, Ramaker, Katrin, Gaede, Karoline, Tappertzhofen, Kristof, Lunding, Lars, Wegmann, Michael, Horbert, Peter, Weber, Karina, Frey, Andreas
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Zdroj: Scientific Reports; 6/4/2024, Vol. 14 Issue 1, p1-12, 12p
Abstrakt: Sudden aggravations of chronic inflammatory airway diseases are difficult-to-foresee life-threatening episodes for which advanced prognosis-systems are highly desirable. Here we present an experimental chip-based fluidic system designed for the rapid and sensitive measurement of biomarkers prognostic for potentially imminent asthma or COPD exacerbations. As model biomarkers we chose three cytokines (interleukin-6, interleukin-8, tumor necrosis factor alpha), the bacterial infection marker C-reactive protein and the bacterial pathogen Streptococcus pneumoniae—all relevant factors in exacerbation episodes. Assay protocols established in laboratory environments were adapted to 3D-printed fluidic devices with emphasis on short processing times, low reagent consumption and a low limit of detection in order to enable the fluidic system to be used in point-of-care settings. The final device demonstrator was validated with patient sample material for its capability to detect endogenous as well as exogenous biomarkers in parallel. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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