Steric hindrance of N6-methyl in m6A and its application for specific loci detection.

Autor: Xiaoyi Xie, Qi Wei, Jingyu Han, Xin Fang, Wei Yang, Xiang Zhou, Yafen Wang, Xiaocheng Weng
Předmět:
Zdroj: Chemical Communications; 4/28/2024, Vol. 60 Issue 33, p4479-4482, 4p
Abstrakt: We found that the N6 methyl group of N6-methyladenine is able to hinder the methylation of N6-methyladenine at the N¹ position by DMS. Based on this, we have devised a novel method for detecting N6-methyladenine, which was successfully applied to identify specific m6A loci in 28S rRNA. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index