Validation of the slaughterhouse porcine heart model for ex-situ heart perfusion studies.
| Autor: | Kaffka genaamd Dengler, Selma E, Mishra, Mudit, van Tuijl, Sjoerd, de Jager, Saskia CA, Sluijter, Joost PG, Doevendans, Pieter A, van der Kaaij, Niels P |
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| Předmět: |
SWINE
BIOLOGICAL models PEARSON correlation (Statistics) EXTRACORPOREAL membrane oxygenation LABORATORY animals T-test (Statistics) RESEARCH funding XENOGRAFTS HEART ORGAN donation MANN Whitney U Test DESCRIPTIVE statistics HEART transplantation FOOD industry KAPLAN-Meier estimator LOG-rank test ANALYSIS of variance PERFUSION DATA analysis software CARDIAC surgery |
| Zdroj: | Perfusion; Apr2024, Vol. 39 Issue 3, p555-563, 9p |
| Abstrakt: | Introduction: To validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6). Methods: All hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20–25 mmHg to achieve a coronary flow between 100–200 mL/min. Reperfusion and testing was performed for 4 hours on a normothermic, oxygenated diluted whole blood loaded heart model. Survival was defined by a cardiac output above 3 L with a mean aortic pressure above 60 mmHg. Results: Both groups showed 100% functional survival, with laboratory hearts displaying superior cardiac function. Both groups showed similar decline in function over time. Conclusion: We conclude that the slaughterhouse heart can be used as an alternative to laboratory hearts and provides a cost-effective method for future ex-situ heart perfusion studies. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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