Maize bran as a potential substrate for production of β-glucosidase.

Autor: Mule, Tejal A., Sawant, Sneha S., Odaneth, Annamma A.
Zdroj: Biomass Conversion & Biorefinery; Feb2024, Vol. 14 Issue 3, p4029-4039, 11p
Abstrakt: Costs and conversion efficiency associated with cellulase preparations have been identified as factors impeding the development of cellulose hydrolysis–based technologies. Supplementing enzymes to fungal cocktails has been practiced; however, the costs associated offset the benefits of an increase in hydrolysis. This work explores the benefits of using maize bran as a substrate in a sequential fermentation mode to produce β-glucosidase (BGL), intended for augmenting commercial fungal cellulases. Aspergillus tubingensis was grown for BGL production on maize bran in sequential solid-state and submerged fermentation (SsF-SmF). In these experiments, a solid substrate was soaked with a nutrient medium and later with media containing 1% solid substrate. The sequential process was observed to yield a two-fold improvement in BGL production in comparison to traditional methods. The predominance of dispersed mycelia in the sequential process was noted and concluded to be the influencer for improved enzyme secretion. Different substrates were evaluated among which delignified maize bran was found to be the most suitable. The batch fermentation yielded BGL productivity of 400 ± 8.64 CBU/l/day, while the repeated fed-batch mode improved the BGL productivity to 647 ± 13.33 CBU/l/day and 987 ± 12.5 CBU/l/day for 50 and 25% medium replacement, respectively. Supplementing commercial cellulases with the produced BGL was observed to increase cellulose hydrolysis by 20%. This work, therefore, helps explore the potential of maize bran as a substrate for sequential SsF-SmF for BGL production in a cost-effective manner. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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