| Abstrakt: |
With the advancement of nanotechnology and the increasing utilization of nanoparticles (NPs), their production and release into the environment are on the rise. Consequently, it is crucial to continuously monitor the toxicity of nanoparticles for humans, animals, and plants, as well as their impact on the environment. This is particularly significant in relation to human health and food production, given the escalating use of nanomaterials in agriculture and horticulture. The aim of the study was to investigate the response of rapeseed seedlings to silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) over different periods of exposure. This research analyzed the impact of these nanoparticles on the biochemical response of rapeseed seedlings after 7, 14, and 21 days of growth in their presence. This study assessed the activity of guaiacol peroxidase (GPOX), pyrogallol peroxidase (PPOX), superoxide dismutase (SOD), and free protein content, as well as the interactions between key elements responsible for oxidative stress and the antioxidant response. The findings demonstrated a significant effect of AgNPs and AuNPs on stimulating the response of rapeseed seedlings, with the activity of PPOX, GPOX, and SOD being dependent on the exposure time and the type and dose of nanoparticles used. Enzyme activity increased with the length of exposure time, while the content of free protein decreased over the weeks. The most intense reaction of seedlings was observed in the case of GPOX, with the lowest activity observed in PPOX and SOD. High effects of the nanoparticle type and rate were also observed in the correlation matrix. This study suggests that a comprehensive analysis of plant reactions to nanoparticles could have a significant impact on the proper and effective use of nanoparticles in agriculture and horticulture. This could lead to the environmentally friendly production of high-quality plant material. [ABSTRACT FROM AUTHOR] |
