| Autor: |
Soldo, Barbara, Bilušić, Tea, Giacometti, Jasminka, Ljubenkov, Ivica, Čikeš Čulić, Vedrana, Bratanić, Andre, Bošković, Perica, Šola, Ivana, Ilić, Krunoslav |
| Předmět: |
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| Zdroj: |
Applied Sciences (2076-3417); Jan2024, Vol. 14 Issue 2, p869, 14p |
| Abstrakt: |
Olive leaves are the richest source of phenolic compounds, particularly oleuropein, which has many beneficial effects on human health. This study compares the effect of three extraction techniques: ultrasonic-solvent extraction, microwave-assisted extraction, and supercritical CO2 extraction of freeze-dried wild olive leaves (Olea europea subps. oleaster, Hoffmanns. & Link) on oleuropein concentration in obtained extracts. The extract with the highest concentration of oleuropein was obtained after low-frequency ultrasound solvent extraction (WOLE-S) (71.97 µg/mL). Oleuropein from that extract showed low stability after simulated gastric digestion with human gastric juices (35.66%) and high stability after 60 min of intestinal phase (65.97%). The highest antiproliferative activity of WOLE-S and pure oleuropein was detected against T24 cancer cells. The antiproliferative activity of WOLE-S against MD-MBA-123, A549, and A1235 cells was moderate, while the activity of oleuropein was high. WOLE-S significantly down-regulated the expression of IL-6 in A549 cells, indicating a possible anti-inflammatory effect in lung cancer cells. The expression of IL-8 was slightly reduced by treating the cells with WOLE-S, while the expression of IL-10 was not altered by the treatment with WOLE-S or oleuropein. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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